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IL-27 Protein (AA 19-228, AA 29-234) (Fc Tag)

This Recombinant IL-27 protein is produced in CHO Cells.
Catalog No. ABIN7566260

Quick Overview for IL-27 Protein (AA 19-228, AA 29-234) (Fc Tag) (ABIN7566260)

Target

See all IL-27 (IL27) Proteins
IL-27 (IL27) (Interleukin 27 (IL27))

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 11
  • 9
  • 1
Human, Mouse

Source

  • 8
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
CHO Cells

Purity

>95 % (SDS-PAGE)
  • Protein Characteristics

    AA 19-228, AA 29-234

    Purification tag / Conjugate

    This IL-27 protein is labelled with Fc Tag.

    Purpose

    IL-27 (mouse):Fc-KIH (human) (rec.)

    Cross-Reactivity

    Human, Mouse

    Characteristics

    IL-27A/p28 (aa 29-234) (mouse):Fc Knobs and IL-27B/EBI3 (aa 19-228) (mouse):Fc Holes form the IL-27 (mouse):Fc-KIH (human) using the Knobs-into-Holes technology (see reference: J.B. Ridgway, et al., Protein Eng. 9, 617 (1996)).

    Endotoxin Level

    <0.01EU/μg purified protein (LAL test).

    Biological Activity Comment

    Activates STAT3 phosphorylation in mouse and human cells.
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  • Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    1 mg/mL after reconstitution.

    Concentration

    1 mg/mL

    Buffer

    Contains PBS

    Handling Advice

    After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0. 1 % BSA should be used for further dilutions.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short Term Storage: +4°C

    Long Term Storage: -20°C

    Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.Working aliquots are stable for up to 3 months when stored at -20°C.

  • Target

    IL-27 (IL27) (Interleukin 27 (IL27))

    Alternative Name

    IL-27

    Background

    IL-27 (mouse):Fc Knobs-into-Holes (human) (rec.), Interleukin-27 Subunit alpha, IL-27 Subunit alpha, IL-27-A, Interleukin-27 Subunit beta, Ebi3, Epstein-Barr Virus-induced Gene 3 Protein Homolog

    Interleukin-12 (IL-12) family members are heterodimer glycoproteins, composed of two covalently linked subunits, alpha and beta chains. The alpha-subunit consists of IL-23p19, IL-27p28, and IL-12p35, and the beta-subunit includes IL-12p40 and Epstein-Barr virus-induced gene (Ebi3). IL-12 members bind to cognate heterodimeric receptor chains expressed on T cells. This family includes IL-12, IL-23, IL-27 and IL-35 and IL-39. IL-12 and IL-23 are predominantly proinflammatory cytokines that contribute key roles in the development of Th1 and Th17 cells, respectively. IL-27 has both pro- and anti-inflammatory properties and is a potent T cell immunomodulator. IL-35, a new member of this family, is a potent inhibitory cytokine produced by natural, thymus-derived regulatory T cell (nTreg) populations. IL-39, the newest member of IL-12 family, mediates the inflammatory response through the activation of STAT1/STAT3 signaling pathway. These IL-12 family members link innate immunity with the development of adaptive immunity and are also important for regulating T cell responses. IL-27 is composed of two subunits, IL-27p28 and Epstein-Barr virus-induced gene 3 (EBI3) signals via a heterodimeric receptor consisting of WSX-1 and glycoprotein (gp130), leading to activation of STAT 1 and 3. IL-27 is pro-inflammatory and promotes NK and T cell proliferation as well as the production of IFN-gamma. It is anti-inflammatory, inhibiting Th2 and Th17 cell activities and stimulating the production of IL-10 by T regulatory cells. IL-27 has potent antiviral activities against numerous viruses, by increasing the production of interferons (IFNs). Finally, IL-27 has antitumor activity as well as anti-angiogenic activity with activation of the production of anti-angiogenic chemokines. The protein IL-27 (mouse):Fc-KIH (human) (rec.) is produced by using two different vectors, one encoding for the IL-27A/p28:Fc Knobs sequence (synthesizing a protein of 62 kDa) and one encoding for the IL27B/EBI3: Fc Holes sequence (synthesizing a protein of 60 kDa). Both vectors transfected into CHO cells produce both Fc molecules (Knobs-into-Holes technology, J.B. Ridgway, et al., Protein Eng. 9, 617 (1996)) required for dimerization and for secretion of the final protein IL-27 (mouse): Fc-KIH (human) (rec.). InVivoKines™ are a new generation of recombinant fusion proteins for immunotherapeutic, preclinical and translational in vivo research

    Molecular Weight

    ~62kDa and 60 kDa (SDS-PAGE)

    UniProt

    Q8K3I6, O35228
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