FNDC5 Protein (AA 29-140, AA 32-143)
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- Target See all FNDC5 Proteins
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 29-140, AA 32-143
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Origin
- Human, Mouse
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Source
- Escherichia coli (E. coli)
- Purpose
- Irisin (rec.) (untagged) (E.coli)
- Cross-Reactivity
- Human, Mouse
- Characteristics
- Irisin (human, aa 32-143 / mouse, aa 29-140) is untagged. Irisin has 100 % identity between mouse, rat, monkey and human.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.1EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product FNDC5 Protein
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Fibronectin Type III Domain Containing 5 (FNDC5) (AA 1-137) protein (GST tag)
FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 15-127) protein (His tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibronectin Type III Domain Containing 5 (FNDC5) protein (His tag)FNDC5 Origin: Human, Mouse, Rat Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 14-78) protein (GST tag)FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 2-137) (Active) protein (His tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) > 90 % AcA, CC Active
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) (Active) protein (His tag)FNDC5 Origin: Human Host: HEK-293 Cells > 97 % AcA, CC Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- 0.1 mg/mL after reconstitution
- Concentration
- 0.1 mg/mL
- Buffer
- Lyophilized from PBS or TRIS buffer (lot-dependent).
- Handling Advice
- After reconstitution, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
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- Target
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- Alternative Name
- Irisin (FNDC5 Products)
- Background
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Fibronectin Type III Domain-containing Protein 5 (cleaved), Fibronectin Type III Repeat-containing Protein 2 (cleaved), FNDC5 (cleaved)
Irisin is a recently described exercise-induced hormone secreted by skeletal muscle in mice and humans. Irisin activates beige fat cells (beige cells have a gene expression pattern distinct from either white or brown fat and are preferentially sensitive to the polypeptide hormone Irisin). Irisin is cleaved from the type I membrane protein FNDC5 and improves systemic metabolism by increasing energy expenditure.
- Molecular Weight
- ~12kDa
- UniProt
- Q8NAU1
- Pathways
- Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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