FNDC5 Protein (AA 32-143) (His tag)
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- Target See all FNDC5 Proteins
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- Biological Activity
- Active
- Protein Characteristics
- AA 32-143
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FNDC5 protein is labelled with His tag.
- Application
- Activity Assay (AcA), Cell Culture (CC)
- Characteristics
- Tag location: N-terminal His Tag
- Purity
- > 97 %
- Biological Activity Comment
- The protein encoded by FNDC5 can be cleaved into Irisin, which is a myokine linked to exercise and lean body mass. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours in at 37oC. Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1. .
- Top Product
- Discover our top product FNDC5 Protein
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Fibronectin Type III Domain Containing 5 (FNDC5) (AA 1-137) protein (GST tag)
FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) protein (His-IF2DI Tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 15-127) protein (His tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 14-78) protein (GST tag)FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) protein (His tag)FNDC5 Origin: Human, Mouse, Rat Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-152) protein (His tag)Crystallography grade FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 29-149) protein (His tag)Crystallography grade FNDC5 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-212) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 29-140) protein (rho-1D4 tag)Crystallography grade FNDC5 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Isoelectric Point: 5
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.6, containing 5 % trehalose.
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- Target
- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
- Alternative Name
- Fibronectin Type III Domain Containing Protein 5 (FNDC5) (FNDC5 Products)
- Synonyms
- FRCP2 Protein, irisin Protein, 1500001L03Rik Protein, AI836596 Protein, C87088 Protein, PeP Protein, Pxp Protein, fndc5 Protein, si:dkey-264p5.1 Protein, zgc:158684 Protein, fibronectin type III domain containing 5 Protein, fibronectin type III domain containing 5b Protein, FNDC5 Protein, Fndc5 Protein, fndc5b Protein
- Background
- Alternative Names: FRCP2, Irisin, Fibronectin type III repeat-containing protein 2
- Molecular Weight
- 27kDa
- UniProt
- Q8NAU1
- Pathways
- Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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