Quick Overview for FNDC5 Protein (His tag) (ABIN7319826)
Target
See all FNDC5 ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This FNDC5 protein is labelled with His tag.
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Purpose
- Recombinant Human/Mouse/Rat Irisin/FNDC5 (N-6His)
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Sequence
- Asp32-Glu143
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Characteristics
- Recombinant Human/Mouse/Rat Fibronectin Type III Domain-containing Protein 5 is produced by our Mammalian expression system and the target gene encoding Asp32-Glu143 is expressed with a 6His tag at the N-terminus.
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Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
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Fibronectin Type III Domain Containing 5 (FNDC5) (AA 1-137) protein (GST tag)
FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 15-127) protein (His-IF2DI Tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 14-78) protein (GST tag)FNDC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) (Active) protein (His tag)FNDC5 Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. WB, SDS, Imm, ELISA, AcA Active
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 2-137) (Active) protein (His tag)FNDC5 Origin: Human Host: Escherichia coli (E. coli) > 90 % AcA, CC Active
Fibronectin Type III Domain Containing 5 (FNDC5) (AA 32-143) (Active) protein (His tag)FNDC5 Origin: Human Host: HEK-293 Cells > 97 % AcA, CC Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please refer to the printed manual for detailed information.
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Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- FNDC5 (Fibronectin Type III Domain Containing 5 (FNDC5))
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Alternative Name
- Irisin/FNDC5
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Background
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Background: Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100 % sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1(UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Synonym: Fibronectin type III domain-containing protein 5, Fibronectin type III repeat-containing protein 2, Irisin, FNDC5
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Molecular Weight
- 13.4 kDa
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UniProt
- Q8NAU1
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Pathways
- Hormone Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Target
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