Histone H3.3 Protein (His tag,thrombin cleavage site)

Catalog No. ABIN7595301

This Recombinant Histone H3.3 protein is produced in Escherichia coli (E. coli).

Quick Overview for Histone H3.3 Protein (His tag,thrombin cleavage site) (ABIN7595301)

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Cytotoxicity Test (CyTox), Staining Methods (StM)

Purity: >95 %

Product Details

Purification tag / Conjugate: This Histone H3.3 protein is labelled with His tag,thrombin cleavage site.

Sequence: HHHHHHLVPR GSMARTKQTA RKSTGGKAPR KQLATKAARK SAPSTGGVKK PHRYRPGTVA LREIRRYQKS TELLIRKLPF QRLVREIAQD FKTDLRFQSA AIGALQEASE AYLVGLFEDT NLCAIHAKRV TIMPKDIQLA RRIRGERA

Specificity: Possible applications in R&D, SDS-PAGE, Westen Blotting

Characteristics: Full length
Homogeneously expressed in E coli
Free of animal and viral components
Shows comparable activity to commercially sourced Histon H3 from Calf Thymus, based on cytotoxicity assay. Supplied in low-binding tubes to ensure optimal sample recovery
Thrombin tag for His-tag removal
This product is for R&D purposes only. Not for human use

Purification: Affinity chromatography

Endotoxin Level: ND

Application Details

Application Notes: SDS PAGE + coomassie blue stain,Cytotoxicity assay,A280

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge tubes before opening. Reconstitute the sample in the water. Avoid pipetting up and down.

Buffer: 25 mM HEPES, 140 mM NaCl pH 7,4

Preservative: Without preservative

Storage: -80 °C

Storage Comment: Stable at -80 °C for at least 6 months in lyophilized form. After reconstitution, if not used, aliquot and store at -80 °C. Avoid repeated freeze and thaw cycles. Lyophilized proteins are shipped at ambient temperature.

Target Details

Target: Histone H3.3 (H3F3A) (H3 Histone, Family 3A (H3F3A))

Alternative Name: Histon H3.3

Background: Histone H3 is a core component for the nucleosome, which is a complex present in chromatin and essential for DNA packaging in the nucleus. It plays a key role in gene regulation through post-translational modifications, influencing processes such as transcription, DNA repair, and cell division.

Molecular Weight: 16,8 kDa

Gene ID: 3021

NCBI Accession: NM_005324

UniProt: P84243