IgG1 Fc (AA 100-330) (Active) Protein

Catalog No. ABIN7596113

Product Details

Target: IgG1 Fc

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 100-330

Origin: Human

Source: HEK-293 Cells

Application: Activity Assay (AcA), SDS-PAGE (SDS)

Sequence: PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K

Purity: > 90% by SDS-PAGE

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102). The ED50 range ≤ 1.5 ug/ml., 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116)., 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098). The ED50 range ≤ 4 ug/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IgG1 Fc

Background: Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a 'Y-shaped' structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc). Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C-terminal half of one heavy chain. Through interaction with its ligands (FcγR, C1q and FcRn) it mediates various immune effector functions and increases the half-life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Molecular Weight: 25.9kDa (231aa)