HAO1 Protein (AA 1-370) (His tag)
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- Target See all HAO1 Proteins
- HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-370
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This HAO1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
- Sequence
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MLPRLVC ISDYEQHVRS VLQKSVYDYY RSGANDQETL ADNIQAFSRW KLYPRMLRNV ADIDLSTSVL GQRVSMPICV GATAMQCMAH VDGELATVRA CQTMGTGMML SSWATSSIEE VAEAGPEALR WMQLYIYKDR EISRQIVKRA EKQGYKAIFV TVDTPYLGNR IDDVRNRFKL PPQLRMKNFE TNDLAFSPKG NFGDNSGLAE YVAQAIDPSL SWDDITWLRR LTSLPIVVKG ILRGDDAKEA VKHGVDGILV SNHGARQLDG VPATIDVLPE IVEAVEGKVE VFLDGGVRKG TDVLKALALG AKAVFVGRPI IWGLAFQGEK GVQDVLEILK EEFRLAMALS GCQNVKVIDK TLVRKNPLAV SKI - Purity
- > 90% by SDS-PAGE
- Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 1,000pmol/min/ug, and defined as the amount of enzyme that oxidize glyoxylate at pH 8.0 at 25C
- Top Product
- Discover our top product HAO1 Protein
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Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) protein (His tag)
custom-made HAO1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) protein (His tag)custom-made HAO1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) protein (His tag)HAO1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) protein (His tag)HAO1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1) (AA 1-370) protein (GST tag)HAO1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- HAO1 (Hydroxyacid Oxidase (Glycolate Oxidase) 1 (HAO1))
- Alternative Name
- Hydroxyacid Oxidase-1/HAO-1 (HAO1 Products)
- Background
- Hydroxyacid Oxidase-1/HAO-1, also known as hydroxyacid oxidase 1, is a member of the superfamily of the alpha hydroxy acid oxidases (HAO) enzymes. It catalyzes the FMN mediated oxidation of glycolate to glyoxylate and glyoxylate to oxalate with reduction of oxygen to hydrogen peroxide. It is most highly expressed in liver and pancreas and is most active on two carbon substrates such as glycolate. Recently, it has been identified as a major contributor to hyperoxaluria, a disorder in which large deposits of calcium oxalate form kidney stones. Recombinant mouse Hydroxyacid Oxidase-1/HAO-1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
- Molecular Weight
- 43.4 kDa (393aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_034533
- Pathways
- Monocarboxylic Acid Catabolic Process
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