Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag)

Catalog No. ABIN7596270

Recombinant Lactate Dehydrogenase A protein expressed in Baculovirus infected Insect Cells.

Quick Overview for Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag) (ABIN7596270)

Target: Lactate Dehydrogenase A (LDHA)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 1-332

Purification tag / Conjugate: This Lactate Dehydrogenase A protein is labelled with His tag.

Sequence: MATLKDQLIV NLLKEEQAPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVMEDKLKG EMMDLQHGSL FLKTPKIVSS KDYCVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNIVKYSPH CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HALSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPELGT DADKEQWKEV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGINEDVFLS VPCILGQNGI SDVVKVTLTP EEEARLKKSA DTLWGIQKEL QF

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 250unit/mg, and is defined as the Amount of enzyme that convert 1.0 umole of pyruvate to L-lactate and per minute at pH 7.5 at 37°C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: Lactate Dehydrogenase A/LDHA

Background: Ldha, also known as L-lactate dehydrogenase A chain isoform 1, is a member of the LDH/MDH superfamily and LDH family. It catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. This protein is found predominantly in muscle tissue. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It plays an important role in the development, invasion and metastasis of malignancies. Recombinant mouse Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Molecular Weight: 37.5 kDa (340aa)

NCBI Accession: NP_034829

Pathways: Warburg Effect