GPT2 Protein (AA 1-522) (His tag)
Quick Overview for GPT2 Protein (AA 1-522) (His tag) (ABIN7596289)
Target
See all GPT2 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-522
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Purification tag / Conjugate
- This GPT2 protein is labelled with His tag.
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Sequence
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QRAAVLVRR GSCPRASGPW GRSHSSAAAE ASAALKVRPE RSPRDRILTL ESMNPQVKAV EYAVRGPIVL KAGEIEMELQ RGIKKPFTEV IRANIGDAHA MGQQPITFLR QVMALCTYPN LLNSPSFPED AKKRARRILQ ACGGNSLGSY SASQGVNCIR EDVAAFITRR DGVPADPDNI YLTTGASDGI STILKLLVSG GGKSRTGVMI PIPQYPLYSA VISELDAVQV NYYLDEENCW ALNVDELRRA LRQAKDHCDP KVLCIINPGN PTGQVQSRKC IEDVIHFAWE EKLFLLADEV YQDNVYSPDC RFHSFKKVLY QMGHEYSSNV ELASFHSTSK GYMGECGYRG GYMEVINLHP EIKGQLVKLL SVRLCPPVSG QAAMDIVVNP PEPGEESFEQ FSREKEFVLG NLAKKAKLTE DLFNQVPGIQ CNPLQGAMYA FPRILIPAKA VEAAQSHKMA PDMFYCMKLL EETGICVVPG SGFGQREGTY HFRMTILPPV DKLKTVLHKV KDFHLKFLEQ YS -
Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 50unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minuteat pH7.5 at 37C.
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Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-522) protein (His tag)
custom-made GPT2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-523) protein (His tag)custom-made GPT2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-523) protein (GST tag)GPT2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (AA 1-523) protein (His tag)GPT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) proteinGPT2 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) protein (His tag)GPT2 Origin: Rat Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GPT2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- GPT2 (Glutamic Pyruvate Transaminase (Alanine Aminotransferase) 2 (GPT2))
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Alternative Name
- ALT2/GPT2
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Background
- ALT2/GPT2, also known as alanine aminotransferase, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. Recombinant mouse ALT2/GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 60.1 kDa (542aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_776291
Target
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