Acetylcholinesterase Protein (AChE) (AA 32-614) (His tag)

Catalog No. ABIN7596291

Recombinant Acetylcholinesterase protein expressed in HEK-293 Cells.

Quick Overview for Acetylcholinesterase Protein (AChE) (AA 32-614) (His tag) (ABIN7596291)

Target: Acetylcholinesterase (AChE)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Purity: > 95% by SDS - PAGE

Product Details

Protein Characteristics: AA 32-614

Purification tag / Conjugate: This Acetylcholinesterase protein is labelled with His tag.

Sequence: EGREDAE LLVTVRGGRL RGIRLKTPGG PVSAFLGIPF AEPPMGPRRF LPPEPKQPWS GVVDATTFQS VCYQYVDTLY PGFEGTEMWN PNRELSEDCL YLNVWTPYPR PTSPTPVLVW IYGGGFYSGA SSLDVYDGRF LVQAERTVLV SMNYRVGAFG FLALPGSREA PGNVGLLDQR LALQWVQENV AAFGGDPTSV TLFGESAGAA SVGMHLLSPP SRGLFHRAVL QSGAPNGPWA TVGMGEARRR ATQLAHLVGC PPGGTGGNDT ELVACLRTRP AQVLVNHEWH VLPQESVFRF SFVPVVDGDF LSDTPEALIN AGDFHGLQVL VGVVKDEGSY FLVYGAPGFS KDNESLISRA EFLAGVRVGV PQVSDLAAEA VVLHYTDWLH PEDPARLREA LSDVVGDHNV VCPVAQLAGR LAAQGARVYA YVFEHRASTL SWPLWMGVPH GYEIEFIFGI PLDPSRNYTA EEKIFAQRLM RYWANFARTG DPNEPRDPKA PQWPPYTAGA QQYVSLDLRP LEVRRGLRAQ ACAFWNRFLP KLLSATDTLD EAERQWKAEF HRWSSYMVHW KNQFDHYSKQ DRCSDL

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 6,000 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1.0 nmol acetylthiocholine per minute at pH7.5 at 25C.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase/ACHE

Background: ACHE, also known as Acetylcholinesterase, is a member of the type-B carboxylesterase/lipase family. It is an enzyme that catalyzes the breakdown of acetylcholine and of some other choline esters that function as neurotransmitters. During neurotransmission, ACH is released from the presynaptic neuron into the synaptic cleft and binds to ACH receptors on the post-synaptic membrane, relaying the signal from the nerve. ACHE, also located on the post-synaptic membrane, terminates the signal transmission by hydrolyzing ACH. This protein is thought to be involved in the pathology of Alzheimers disease (AD) by accelerating the assembly of A beta peptides into fibrillar species through forming complexes with A beta via the peripheral anionic site on ACHE. ACHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance ACH availability, as well as reduce amyloidogenesis and subsequent neurotoxicity. Recombinant human ACHE, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Molecular Weight: 65.6kDa (592aa)

NCBI Accession: NP_000656

Pathways: Skeletal Muscle Fiber Development