HYAL1 Protein (AA 22-435) (His tag)

Catalog No. ABIN7596385

This Recombinant HYAL1 protein is produced in HEK-293 Cells.

Quick Overview for HYAL1 Protein (AA 22-435) (His tag) (ABIN7596385)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: > 90% by SDS-PAGE

Product Details

Protein Characteristics: AA 22-435

Purification tag / Conjugate: This HYAL1 protein is labelled with His tag.

Sequence: FRGPLLPNRP FTTVWNANTQ WCLERHGVDV DVSVFDVVAN PGQTFRGPDM TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD FSGLAVIDWE AWRPRWAFNW DTKDIYRQRS RALVQAQHPD WPAPQVEAVA QDQFQGAARA WMAGTLQLGR ALRPRGLWGF YGFPDCYNYD FLSPNYTGQC PSGIRAQNDQ LGWLWGQSRA LYPSIYMPAV LEGTGKSQMY VQHRVAEAFR VAVAAGDPNL PVLPYVQIFY DTTNHFLPLD ELEHSLGESA AQGAAGVVLW VSWENTRTKE SCQAIKEYMD TTLGPFILNV TSGALLCSQA LCSGHGRCVR RTSHPKALLL LNPASFSIQL TPGGGPLSLR GALSLEDQAQ MAVEFKCRCY PGWQAPWCER KSMW

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: Hyaluronidase 1/HYAL1

Background: HYAL1, also known as Hyaluronidase-1, is a member of the endolytic glycoside hydrolase family. Human hyaluronidases (HYALs) are a group of five endo-β-N-acetyl-hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20). HYAL1 preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. This protein is active at an acidic pH and is the major hyaluronidase in plasma. Defects in HYAL1 are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. It is implicated in several types of cancers, likely due to the angiogenic effects of HYAL1-cleaved hyaluronan fragments. Recombinant human HYAL1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Molecular Weight: 46.9kDa (420aa)

NCBI Accession: NP_149349

Pathways: Glycosaminoglycan Metabolic Process