KIAA0101 Protein (AA 1-111) (His tag)
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- Target See all KIAA0101 (PAF) Proteins
- KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-111
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This KIAA0101 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
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MVRTKAD SVPGTYRKVV AARAPRKVLG SSTSATNSTS VSSRKAENKY AGGNPVCVRP TPKWQKGIGE FFRLSPKDSE KENQIPEEAG SSGLGKAKRK ACPLQPDHTN DEKE - Purity
- > 90% by SDS-PAGE
- Top Product
- Discover our top product PAF Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- Target
- KIAA0101 (PAF) (PCNA Clamp Associated Factor (PAF))
- Alternative Name
- KIAA0101/PCLAF (PAF Products)
- Background
- KIAA0101 is a PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. This protein also acts as a regulator of centrosome number. Recombinant human KIAA0101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 14.4 kDa (134aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_055551
- Pathways
- Chromatin Binding
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