IgE Protein
Quick Overview for IgE Protein (ABIN7596472)
Target
See all IgE ProteinsProtein Type
Origin
Source
Application
Purity
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Clonality
- Chimeric
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Purpose
- Chimeric Human IgE (JW8)
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Specificity
- 4-Hydroxy-3-nitrophenylacetyl (NP)
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Characteristics
- Recombinant chimeric IgE consisting of a human Fc heavy chain linked to the antigen binding variable region of a mouse hybridoma by in vitro DNA recombination. The antibody is specific for the hapten 4-Hydroxy-3-iodo-5-nitrophenylacetyl (NIP). IgE (JW8) recognizes the human high affinity IgE receptor (FceRIa) and does not interact with the corresponding IgE receptors of other species. It can be used as functional reagent in mast cell / basophil cellular assays, e.g. for controlled sensitization of such cells and subsequent cross-linking with NIP-BSA conjugate as specific cross-linking agent.
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Purification
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B – mouse anti-human IgE mAb (BSW17)
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Sterility
- Sterile filtered
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IgE (AA 208-429) protein (His-Avi Tag)
Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 209-428) protein (His-Avi Tag)Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
IgE (AA 91-421) protein (His tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE (AA 198-421) protein (His-Avi Tag)Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
IgE proteinOrigin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE protein (Biotin)Origin: Human Host: Human Recombinant > 95 %, tested by analytical Gel Permeation Chromatography WB, ELISA, IsoC, Func, FACS
IgE (AA 1-428) protein (His tag)Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-432) protein (His tag)Origin: Cynomolgus Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
IgE (AA 1-429) protein (His tag)Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % WB, SDS, Imm, PC
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Application Notes
- ELISA, Flow Cytometry, functional cellular assays (mast cell / basophil activation).
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Comment
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100% reactivity, compared to calibrator (Internal Standard IgE (JW8)), tested by SPR (Biacore™) binding to immobilized recombinant human high affinity IgE receptor
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Phosphate buffered saline (PBS) pH = 7.2
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Preservative
- Azide free
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Storage
- 4 °C
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Storage Comment
- Store undiluted at 4 °C. For long term storage, aliquot and store at -20°C or -80°C. Avoid repeated freeze-thawing. Handle under sterile conditions, the mAb is formulated in sterile buffer solution and does not contain sodium azide as preservative. The product may be shipped on ice pack or dry ice.
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- IgE
Target
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