Claudin 3 Protein (CLDN3) (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7596559

This Synthetic Nanodisc Claudin 3 protein is produced in HEK-293 Cells.

Quick Overview for Claudin 3 Protein (CLDN3) (DYKDDDDK Tag,Strep Tag) (ABIN7596559)

Target: Claudin 3 (CLDN3)

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Application: Immunogen (Imm), ELISA, Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Product Details

Purification tag / Conjugate: This Claudin 3 protein is labelled with DYKDDDDK Tag,Strep Tag.

Purpose: Human CLDN3-Strep full length protein-synthetic nanodisc

Application Details

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins
• Mammalian cell expression system ensures post- translational modifications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Claudin 3 (CLDN3)

Alternative Name: CLDN3

Background: C7orf1, CPE-R2, CPETR2, HRVP1, RVP1
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. [provided by RefSeq, Jul 2008]

Molecular Weight: The human full length CLDN3-Strep protein has a MW of 23.3 kDa

UniProt: O15551

Pathways: Hepatitis C