Muscarinic Acetylcholine Receptor M2 Protein (DYKDDDDK Tag,Strep Tag)
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- Target See all Muscarinic Acetylcholine Receptor M2 (CHRM2) Proteins
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
- Protein Type
- Synthetic Nanodisc
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Muscarinic Acetylcholine Receptor M2 protein is labelled with DYKDDDDK Tag,Strep Tag.
- Application
- Immunogen (Imm), ELISA, Cryogenic electron microscopy (cryo-EM), Phage Display (PhD), Surface Plasmon Resonance (SPR)
- Purpose
- Human CHRM2-Strep full length protein-synthetic nanodisc
- Top Product
- Discover our top product CHRM2 Protein
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Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 1-466) protein (His tag)
custom-made CHRM2 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 1-466) protein (His tag)custom-made CHRM2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 1-22) protein (Fc Tag)CHRM2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Cholinergic Receptor, Muscarinic 2 (CHRM2) (AA 251-350) protein (GST tag)CHRM2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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- Comment
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Mammalian cell expression system ensures post- translational modifications
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- Expiry Date
- 12 months
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- Target
- Muscarinic Acetylcholine Receptor M2 (CHRM2) (Cholinergic Receptor, Muscarinic 2 (CHRM2))
- Alternative Name
- CHRM2 (CHRM2 Products)
- Background
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HM2
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 2 is involved in mediation of bradycardia and a decrease in cardiac contractility. Multiple alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008] - Molecular Weight
- The human full length CHRM2-Strep protein has a MW of 51.7 kDa
- UniProt
- P08172
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