GPR31 Protein (DYKDDDDK Tag,Strep Tag)
Quick Overview for GPR31 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7596779)
Target
See all GPR31 ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This GPR31 protein is labelled with DYKDDDDK Tag,Strep Tag.
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Purpose
- Human GPR31-Strep full length protein-synthetic nanodisc
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G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (His tag)
custom-made GPR31 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (His tag)custom-made GPR31 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
G Protein-Coupled Receptor 31 (GPR31) (AA 1-319) protein (GST tag)GPR31 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Comment
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Mammalian cell expression system ensures post- translational modifications
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- GPR31 (G Protein-Coupled Receptor 31 (GPR31))
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Alternative Name
- GPR31
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Background
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12-HETER, HETER, HETER1
High-affinity receptor for 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (12-S-HETE). 12-(S)-HETE is an arachidonic acid metabolite secreted by platelets and tumor cells, and known to induce endothelial cells retraction allowing invasive cell access to the subendothelial matrix, which is a critical step for extravasation or metastasis. Ligand-binding lead to activation of ERK1/2 (MAPK3/MAPK1), MEK, and NF-kappa-B.[UniProtKB/Swiss-Prot Function] -
Molecular Weight
- The human full length GPR31-Strep protein has a MW of 35.1 kDa
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UniProt
- O00270
Target
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