KCTD11 Protein (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7597083

Product Details

Target: KCTD11 (Potassium Channel Tetramerisation Domain Containing 11 (KCTD11))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This KCTD11 protein is labelled with DYKDDDDK Tag,Strep Tag.

Application: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Purpose: Human KCD11-Strep full length protein-synthetic nanodisc

Application Details

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins
• Mammalian cell expression system ensures post- translational modifications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: KCTD11 (Potassium Channel Tetramerisation Domain Containing 11 (KCTD11))

Alternative Name: KCD11 (KCTD11 Products)

Background: C17orf36, KCASH1, REN, REN/KCTD11
Plays a role as a marker and a regulator of neuronal differentiation, Up-regulated by a variety of neurogenic signals, such as retinoic acid, epidermal growth factor/EGF and NGFB/nerve growth factor. Induces apoptosis, growth arrest and the expression of cyclin-dependent kinase inhibitor CDKN1B. Plays a role as a tumor repressor and inhibits cell growth and tumorigenicity of medulloblastoma (MDB). Acts as probable substrate-specific adapter for a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex towards HDAC1. Functions as antagonist of the Hedgehog pathway on cell proliferation and differentiation by affecting the nuclear transfer of transcription factor GLI1, thus maintaining cerebellar granule cells in undifferentiated state, this effect probably occurs via HDAC1 down-regulation, keeping GLI1 acetylated and inactive. When knock-down, Hedgehog antagonism is impaired and proliferation of granule cells is sustained. Activates the caspase cascade.[UniProtKB/Swiss-Prot Function]

Molecular Weight: The human full length KCD11-Strep protein has a MW of 25.9 kDa

UniProt: Q693B1

Pathways: Hedgehog Signaling