P2RX4 Protein (DYKDDDDK Tag,Strep Tag)
Quick Overview for P2RX4 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7597196)
Target
See all P2RX4 ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This P2RX4 protein is labelled with DYKDDDDK Tag,Strep Tag.
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Purpose
- Human P2RX4-Strep full length protein-synthetic nanodisc
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Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4) (AA 1-388) protein (His tag)
custom-made P2RX4 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4) (AA 1-388) protein (His tag)custom-made P2RX4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4) (AA 1-388) protein (GST tag)P2RX4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4) protein (Myc-DYKDDDDK Tag)P2RX4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Comment
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Mammalian cell expression system ensures post- translational modifications
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- P2RX4 (Purinergic Receptor P2X, Ligand-Gated Ion Channel, 4 (P2RX4))
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Alternative Name
- P2RX4
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Background
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P2X4, P2X4R
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012] -
Molecular Weight
- The human full length P2RX4-Strep protein has a MW of 43.4 kDa
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UniProt
- Q99571
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Pathways
- Synaptic Membrane
Target
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