APOA1 Protein (AA 19-267) (His tag)

Catalog No. ABIN7597238

This Recombinant APOA1 protein is expressed in HEK-293 Cells.

Quick Overview for APOA1 Protein (AA 19-267) (His tag) (ABIN7597238)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 19-267

Purification tag / Conjugate: This APOA1 protein is labelled with His tag.

Purpose: Recombinant human APOA1 Protein with C-terminal 10xHis tag

Sequence: APOA1(Arg19-Gln267) 10xHis tag

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1

Background: AMYLD3, HPALP2, apo(a)
This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The encoded preproprotein is proteolytically processed to generate the mature protein, which promotes cholesterol efflux from tissues to the liver for excretion, and is a cofactor for lecithin cholesterolacyltransferase (LCAT), an enzyme responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein. [provided by RefSeq, Dec 2015]

Molecular Weight: predicted molecular mass of 30.3 kDa after removal of the signal peptide.

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism