P-Cadherin Protein (CDH3) (AA 24-655) (His tag)

Catalog No. ABIN7597285

Product Details

Target: P-Cadherin (CDH3)

Protein Type: Recombinant

Protein Characteristics: AA 24-655

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This P-Cadherin protein is labelled with His tag.

Purpose: Recombinant Cynomolgus CDH3 protein with C-terminal 10xHis tag

Sequence: CDH3(Ser24-Phe655) 10xHis tag

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: P-Cadherin (CDH3)

Alternative Name: CDH3 (CDH3 Products)

Background: CDHP, HJMD, PCAD
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene are associated with hypotrichosis with juvenile macular dystrophy and ectodermal dysplasia, ectrodactyly, and macular dystrophy syndrome (EEMS). [provided by RefSeq, Nov 2015]

Molecular Weight: predicted molecular mass of 70.9 kDa after removal of the signal peptide. The apparent molecular mass of cCDH3-His is 70-130 kDa due to glycosylation.

NCBI Accession: XP_005592414