ITGAV/ITGB5 Protein (AA 31-992) (His tag,Fc Tag)

Catalog No. ABIN7597404

Product Details

Target: ITGAV/ITGB5 (Integrin alpha V, Integrin beta 5 (ITGAV/ITGB5))

Protein Type: Recombinant

Protein Characteristics: AA 31-992

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ITGAV/ITGB5 protein is labelled with His tag,Fc Tag.

Purpose: Recombinant human ITGAV protein with C-terminal 6xHis tag and human ITGB5 protein with C-terminal human Fc tag

Sequence: ITGAV(Phe31-Val992) 6xHis tag and ITGB5(Gly24-Asn719) hFc(Glu99-Ala330)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was made using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB5 (Integrin alpha V, Integrin beta 5 (ITGAV/ITGB5))

Alternative Name: ITGAV and ITGB5 (ITGAV/ITGB5 Products)

Background: CD51, MSK8, VNRA, VTNR and ITB5, Integrin beta-5
Integrin alpha V beta 5 (ITGAV & ITGB5) is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, embryonic stem cells, and select endothelium and epithelium. ITGAV & ITGB5 binds ligands containing an RGD motif, notably vitronectin. Growth factors that increase PKC activity, such as VEGF or TGF alpha, promote ITGAV & ITGB5-mediated angiogenesis while alpha V beta 3, which may be expressed in the same cell, responds to FGF-basic and TNF alpha. An inhibitor of both down regulates tumor angiogenesis. During lung inflammation, up regulation of ITGAV & ITGB5 on myofibroblasts or infiltrating lymphocytes may contribute to fibrosis by freeing TGF beta from latency.

Molecular Weight: predicted molecular mass of 107.1 and 102.7 kDa after removal of the signal peptide.

UniProt: P06756, P18084