SMPD1 Protein (AA 62-628) (His tag)

Catalog No. ABIN7607352

Recombinant SMPD1 protein expressed in Mammalian Cells.

Quick Overview for SMPD1 Protein (AA 62-628) (His tag) (ABIN7607352)

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 62-628

Purification tag / Conjugate: This SMPD1 protein is labelled with His tag.

Purpose: Recombinant Human SMPD1/aSMase/ASM Protein, C-10*His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to cleave 2-NHexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,700 pmol/min/μg, as measured under the described conditions.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Alternative Name: aSMase

Background: ASMase, SMPD1, Acid sphingomyelinase, Sphingomyelin phosphodiesterase, ASM

UniProt: P17405