SFRP2 Protein (AA 25-295) (His tag)
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- Target See all SFRP2 Proteins
- SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 25-295
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Origin
- Mouse
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Source
- Mammalian Cells
- Purification tag / Conjugate
- This SFRP2 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)
- Purpose
- Recombinant Mouse SFRP2 Protein, C-His (Active)
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- <0.1 EU/μg of the protein by the LAL method.
- Biological Activity Comment
- Measured by its ability to compete with Frizzled-1 for binding to biotinylated Wnt-3a. The IC50 value is 0.7-3.5 nM, under conditions in which recombinant Mouse Frizzled-1 is present at 2.1 nM, and biotinylated rmWnt-3a concentration is 2.7 nM.
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Secreted Frizzled-Related Protein 2 (SFRP2) (AA 25-295) protein (His tag)
SFRP2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Secreted Frizzled-Related Protein 2 (SFRP2) (AA 25-295) protein (His tag)SFRP2 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Secreted Frizzled-Related Protein 2 (SFRP2) (AA 25-171) protein (His-IF2DI Tag)SFRP2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.
- Expiry Date
- 12 months
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- Target
- SFRP2 (Secreted Frizzled-Related Protein 2 (SFRP2))
- Alternative Name
- Secreted frizzled-related protein 2 (SFRP2 Products)
- Background
- Secreted frizzled-related protein 2, FRP-2, sFRP-2, Secreted apoptosis-related protein 1, SARP-1, SFRP2, FRP2, SARP1, FKSG12, UNQ361/PRO697
- UniProt
- P97299
- Pathways
- WNT Signaling, Tube Formation, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Positive Regulation of fat Cell Differentiation
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