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NOTUM Protein (AA 81-451, C330S) (His tag)

Recombinant NOTUM protein expressed in Mammalian Cells.
Catalog No. ABIN7607619

Quick Overview for NOTUM Protein (AA 81-451, C330S) (His tag) (ABIN7607619)

Target

See all NOTUM Proteins
NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

Protein Type

Recombinant

Biological Activity

Active

Origin

Human

Source

  • 1
  • 1
Mammalian Cells

Application

ELISA, Western Blotting (WB), Activity Assay (AcA), Immunogen (Imm), SDS-PAGE (SDS)

Purity

>90 % as determined by SDS-PAGE.
  • Protein Characteristics

    AA 81-451, C330S

    Purification tag / Conjugate

    This NOTUM protein is labelled with His tag.

    Purpose

    Recombinant Human NOTUM Protein, C-His (Active)

    Endotoxin Level

    <0.1 EU/μg of the protein by the LAL method.

    Biological Activity Comment

    Measured by its ability to inhibit Wnt induced TCF reporter activity in human embryonic kidney cells (HEK293). The ED50 for this effect is 0.1-0.6 μg/mL in the presence of 0.5 μg/mL of recombinant human Wnt-3a.
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  • Application Notes

    Optimal working dilution should be determined by the investigator.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

    Storage

    4 °C,-20 °C,-80 °C

    Storage Comment

    Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

    Expiry Date

    12 months
  • Target

    NOTUM (Notum Pectinacetylesterase Homolog (NOTUM))

    Alternative Name

    NOTUM

    Background

    NOTUM, hNOTUM, Palmitoleoyl-protein carboxylesterase NOTUM

    UniProt

    Q6P988
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