MEPE Protein (AA 18-525) (His tag)

Catalog No. ABIN7607741

Product Details

Target: MEPE (Matrix Extracellular phosphoglycoprotein (MEPE))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 18-525

Origin: Human

Source: Mammalian Cells

Purification tag / Conjugate: This MEPE protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purpose: Recombinant Human MEPE/OF45 Protein, C-10*His (Active)

Purity: >90 % as determined by SDS-PAGE.

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to induce adhesion of mouse chondrogenic cells (ATDC5). rhMEPE/OF45 (Cat #: AHJ64401) immobilized at 2 μg/mL (100 μL/well), can induce more than 60% of ATDC5 cell adhesion.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: MEPE (Matrix Extracellular phosphoglycoprotein (MEPE))

Alternative Name: MEPE (MEPE Products)

Background: MEPE, OF45, Osteoregulin, Osteoblast/osteocyte factor 45, Matrix extracellular phosphoglycoprotein

UniProt: Q9NQ76