FIL1d Protein (AA 2-155)

Catalog No. ABIN7668988

This Recombinant FIL1d protein is produced in Escherichia coli (E. coli).

Quick Overview for FIL1d Protein (AA 2-155) (ABIN7668988)

Target: FIL1d (Interleukin 1 delta (FIL1d))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 2-155

Purpose: Recombinant Human IL-36 Receptor Antagonist Protein/IL-36RN/IL-1F5

Sequence: Val2-Asp155

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma-induced IL-8 secretion in A431 human epithelial carcinoma cells. The ED₅₀ for this effect is 23.14 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: FIL1d (Interleukin 1 delta (FIL1d))

Alternative Name: IL-36RN,IL-1F5

Background: Human Interleukin-36 Receptor Antagonist (IL-36RN) is a secreted protein which belongs to the Interleukin 1 cytokine family (IL-1 family). IL-36RN is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL-36RN is also detected in the spleen, brain leukocyte and macrophage cell types. Increased in lesional psoriasis skin. IL-36RN is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to Interleukin 1 family member 9 (IL1F9). Dysregulated expression of novel agonistic and antagonistic IL-1 family member ligands can promote cutaneous inflammation, revealing potential novel targets for the treatment of inflammatory skin disorders. Human and mouse IL-36RN share 90 % sequence identity.,FIL,UNQ,PRO,IL1F,IL1L,IL1HY,IL1RP,PSORS,IL36RN,FIL1,FIL1(DELTA),FIL1D,IL-36Ra,IL1F5,IL1HY1,IL1L1,IL1RP3,IL36RA,PSORP,PSORS14,UNQ1896,PRO4342,FIL1 Delta,IL-1 Delta,IL-1F5,IL-1HY1,IL-1L1,IL-1ra Homolog 1,IL-1-Related Protein 3,IL-1RP3,Interleukin-1 Delta,Interleukin-1 Family Member 5,Interleukin-1 HY1,Interleukin-1 Receptor Antagonist Homolog 1,Interleukin-1-Like Protein 1,Interleukin-36 Receptor Antagonist Protein

Molecular Weight:

calculated_mw: 16.8 kDa

observed_mw: 17 kDa

UniProt: Q9UBH0