Fibronectin Protein

Catalog No. ABIN7680466

This Recombinant Fibronectin protein is produced in Rice (Oryza sativa).

Quick Overview for Fibronectin Protein (ABIN7680466)

Target: Fibronectin

Protein Type: Recombinant

Origin: Human

Source: Rice (Oryza sativa)

Purity: More than 95 % as determined by SDS-PAGE. 

Grade: Animal-Free

Product Details

Purpose: OsrhFN - Recombinant Human Fibronectin

Characteristics: The recombinant Osrh products are expressed in the rice endosperm platform, which is animal-free and has high purity. It avoids the risk of contamination from human or animal-derived viruses and mycoplasma. The rice endosperm platform has high scalability.This product arrives as White lyophilized powder 

Endotoxin Level: Less than 0.5 EU/ug

Application Details

Application Notes: OsrhFN is generally used in the concentration range of 1-5 micrograms per cm2 of growth surface for attachment or at 5-50 micrograms per ml as a media additive.

(Optimal working dilutions must be determined by end user.)

Comment:

The recombinant human fibronectin (OsrhFN) was expressed in the rice endosperm platform, which is animal component free and has high purity, and has been demonstrated has the same physical and chemical with the plasma derived Fn. OsrhFN provides a safety solution to replace the plasma derived FN.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For the lyophilized OsrhFN, add sterile 1 mL of Sodium Citrate solution (20 mM Citrate, 250 mM NaCl, pH 7.20) to the vial to reconstitute the Fibronectin (recommended reconstituted concentration is 1 mg/mL), shake gently to dissolve the lyophilized powder. The reconstituted OsrhFN liquid can be made in other aqueous buffer.

Handling Advice: Use as soon as possible after opening. Avoid freeze-thaw cycles

Storage: -20 °C

Storage Comment: Stored at temperatures -20°C for at least 36 months.  Upon reconstitution OsrhFN should be stored below -20°C. Use as soon as possible after opening. Please avoid freeze-thaw cycles.

Expiry Date: 36 months

Target Details

Target: Fibronectin

Background: Fibronectin (FN) is a particularly important and well-studied component of the extracellular matrix, and is known to play a key role in cell adhesion, growth, spreading, migration, differentiation and proliferation. Fn is a 200-250 kDa glycoprotein composed of 2 subunits bound via a disulfide bond. Currently, the Fn is purified from the plasma, which however is limited by the availability of supply.