Streptavidin Protein (Cy5)

Catalog No. ABIN7764574

Native Streptavidin protein expressed in Streptomyces avidinii.

Quick Overview for Streptavidin Protein (Cy5) (ABIN7764574)

Target: Streptavidin

Protein Type: Native

Origin: Streptomyces avidinii

Source: Streptomyces avidinii

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), FLISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Multiplex Assay (MA), Dot Blot (DB), Immunofluorescence (IF), Microarray (MA), Fluorescence in situ hybridization (FISH), Immunoelectron Microscopy (IEM), Functional Studies (Func)

Product Details

Purification tag / Conjugate: This Streptavidin protein is labelled with Cy5.

Purpose: Streptavidin Cy5 Conjugated

Purification: Cy5 conjugated Streptavidin was prepared from chromatographically purified streptavidin followed by conjugation to the dye Cy5 and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc aga

Application Details

Application Notes:

Flow Cytometry Dilution: 1:500 - 1:2,500

Application Note: Streptavidin Cy5 Conjugated has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, in

FLISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable Expiration date is one (1) year from date of receipt.

Expiry Date: 12 months

Target Details

Target: Streptavidin

Background:

Synonyms: SA, S avidin, streptococcus avidin, Streptavidin Cy5 Conjugated, Cy5 conjugated Streptavidin

Background: Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, im