HIV1RT Protein
HIV1RT
Origin: Human Immunodeficiency Virus (HIV)
Host: Escherichia coli (E. coli)
Recombinant
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- Target See all HIV1RT products
- HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))
- Protein Type
- Recombinant
- Origin
- Human Immunodeficiency Virus (HIV)
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Source
- Escherichia coli (E. coli)
- Characteristics
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Purified recombinant HIV1-RT protein
Expression System: E.coli
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HIV-1 Reverse Transcriptase (HIV1RT) (Active) protein
HIV1RT Origin: Human Immunodeficiency Virus (HIV) Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS-PAGE (CBB staining) DB, Func, SDS, ELISA, WB Active
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in sterile distilled water.
- Buffer
- Supplied lyophilized from PBS buffer, with no preservatives added,
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -70 °C for long term storage.
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- Target
- HIV1RT (HIV-1 Reverse Transcriptase (HIV1RT))
- Alternative Name
- HIV1-RT (HIV1RT Products)
- Target Type
- Viral Protein
- Background
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HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
Alternative Names: HIV-1 RT, HIV 1-RT, HIV-1 RT protein, HIV1RT protein, HIV1 RT protein, HIV 1-RT protein, HIV-1 Reverse Transcriptase protein, HIV1-RT
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