PNMT Protein (AA 1-282)
Quick Overview for PNMT Protein (AA 1-282) (ABIN935067)
Target
See all PNMT ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-282
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Sequence
- MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL
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Characteristics
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Purified recombinant Human PNMT protein
Expression System: E.coli
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Phenylethanolamine N-Methyltransferase (PNMT) (AA 1-282) protein (His tag)
custom-made PNMT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phenylethanolamine N-Methyltransferase (PNMT) (AA 1-295) protein (His tag)custom-made PNMT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Phenylethanolamine N-Methyltransferase (PNMT) (AA 1-282) protein (GST tag)PNMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phenylethanolamine N-Methyltransferase (PNMT) protein (Myc-DYKDDDDK Tag)PNMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- PNMT protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- PNMT (Phenylethanolamine N-Methyltransferase (PNMT))
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Alternative Name
- PNMT
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Background
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PNMT, also known as phenylethanolamine N-methyltransferase, is an enzyme found in the adrenal medulla that catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Recombinant PNMT protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: PNMTase protein, Phenylethanolamine N-methyltransferase '', PENT protein, Noradrenaline-N-methyltransferase protein -
Molecular Weight
- 30.8 Da (282 AA)
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Pathways
- Response to Water Deprivation
Target
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