HRG1 beta (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 98 % pure
Active
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- Target
- HRG1 beta
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Sequence
- SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE
- Characteristics
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Purified recombinant Human HRG1 beta protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ? 0.5 ng/mL, corresponding to a specific activity of ? 2 x 10^6 units/mg. - Purity
- > 98 % pure
- Endotoxin Level
- < 0.1 ng per μg (1 EU/μg).
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Comment
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Biological activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is approx. 0.5 ng/mL, corresponding to a specific activity of approx. 2 x 10^6 units/mg.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL.
- Buffer
- Supplied lyophilized with no additives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
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- Target
- HRG1 beta
- Background
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Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-beta1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation.
Alternative Names: Neuregulin1 protein, HRG beta 1 protein, HRG1 beta, Neu differentiation factor protein, HRG beta-1 protein, HRG beta 1, HRG beta-1, Heregulin 1 protein, NRG1-beta1 protein
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