HRG1 beta (Active) Protein

Catalog No. ABIN935431

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for HRG1 beta (Active) Protein (ABIN935431)

Target: HRG1 beta

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYKHLGIE FMEAE

Characteristics: Purified recombinant Human HRG1 beta protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is ? 0.5 ng/mL, corresponding to a specific activity of ? 2 x 10^6 units/mg.

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is approx. 0.5 ng/mL, corresponding to a specific activity of approx. 2 x 10^6 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL.

Buffer: Supplied lyophilized with no additives.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: HRG1 beta

Background: Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-beta1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation.
Alternative Names: Neuregulin1 protein, HRG beta 1 protein, HRG1 beta, Neu differentiation factor protein, HRG beta-1 protein, HRG beta 1, HRG beta-1, Heregulin 1 protein, NRG1-beta1 protein