beta Defensin 1 Protein

Catalog No. ABIN987768

This beta Defensin 1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for beta Defensin 1 Protein (ABIN987768)

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % by SDS-PAGE and HPLC analyses.

Product Details

Sequence: GNFLTGLGHR SDHYNCVSSG GQCLYSACPI FTKIQGTCYR GKAKCC

Characteristics: Fully biologically active when compared to standard. The ED50 determined by a chemotaxis bioassay usingCD34+ dendritic cells is less than 1000 ng/ml, corresponding to a specific activity of >, 1.0 × 103 IU/mg.

Endotoxin Level: Level Less than 1EU/µg of rHuBD-1 as determined by LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage: 4 °C

Target Details

Target: beta Defensin 1 (DEFB1) (Defensin, beta 1 (DEFB1))

Alternative Name: beta-Defensin 1 (BD-1)

Background: Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The ª-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified, BD-1, BD-2, BD-3 and BD-4. beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Synonym: beta-Defensin 1 (BD-1), Human. Formulation: Lyophilized from a 0.2µm filtered concentrated solution in 20mM PB, pH 7.4, 130mM NaCl.

Molecular Weight: Approximately 5.0 KDa, a single non-glycosylated polypeptide chain containing 47 amino acids.