G-CSF Protein

Catalog No. ABIN987878

Recombinant G-CSF protein expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for G-CSF Protein (ABIN987878)

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % as determined by:(a) Analysis by SEC-HPLC(b)Analysis by reducing and non-reducing SDS-PAGE silver-stained gel

Product Details

Sequence: analysis The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met-Thr-Pro-Leu-Gly-Pro-Ala-Ser-Ser-Leu-Pro-Gln-Ser-Phe-Leu-Leu. p to grams level.

Characteristics: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to aof 6.0×107 IU/mg

Endotoxin Level: Level Less than 0.03ng/µg (0.3 IEU/µg) determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended to reconstitute the lyophilized Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) in sterile 18 M-omega-cm H2O not less than 100 myg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.815 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a standard solution of recombinant G-CSF as a Reference Standard.

Storage: -20 °C

References

Xie, Gorodetsky, Micewicz, Micevicz, Mackenzie, Gaberman, Levdansky, McBride: "Marrow-derived stromal cell delivery on fibrin microbeads can correct radiation-induced wound-healing deficits." in: The Journal of investigative dermatology, Vol. 133, Issue 2, pp. 553-61, (2013) (PubMed).

Target Details

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Alternative Name: Granulocyte Colony Stimulating Factor (G-CSF)

Background: Human Granulocyte Colony Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant Human Granulocyte Colony Stimulating Factor (G-CSF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18,800 Da. Synonym: rHuG-CSF, G-CS. Formulation: The protein was lyophilized after extensive dialysis against 10mM HAc-NaAc, pH4.0, 150mM NaCl, 0.004% Tween 80, 5% Mannitol buffer.

Molecular Weight: 18,800 Da Isoelectric Point The main zone between 5.8-6.6 analysis by IEF UV Scan The maximal absorption wave is 278+/-3 nm.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization