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Protein A, protein G and protein L

Antibody purification using antibody-binding proteins

Written/Edited by Rene von der Forst, MSE

Protein A, protein G, and Protein L are native and recombinant proteins of microbial origin that bind to mammalian immunoglobulins (Ig). Protein A and protein G bind with high affinity and specificity to the Fc region of immunoglobulins from various mammalian species, most notably the IgGs. Protein L binds immunoglobulins via the kappa light chain. These antibody-binding proteins can be used to purify, immobilize or detect immunoglobulins. These proteins (i.e. protein A, protein G, protein L) are typically attached onto a solid support such as sepharose or magnetic beads. Discover the various formats of antibody-binding proteins available at antibodies-online down below.

Available Antibody-binding Proteins and Beads

Product
Cat. No.
Application
Quantity
Delivery
Datasheet
Cat. No. ABIN376986
Application ELISA, IP
Quantity 5 mg
Delivery 3 to 4 Days
Datasheet Datasheet
Cat. No. ABIN964524
Application IP
Quantity 2 mL
Delivery 1 to 2 Days
Datasheet Datasheet
Cat. No. ABIN1118464
Application IP
Quantity 2 mL
Delivery 1 to 2 Days
Datasheet Datasheet
Product
Cat. No.
Application
Quantity
Delivery
Datasheet
Cat. No. ABIN400575
Application IP, Purif, AC
Quantity 4 mL
Delivery 4 to 6 Days
Datasheet Datasheet
Cat. No. ABIN400576
Application Purif, IP, AC
Quantity 2 mL
Delivery 4 to 6 Days
Datasheet Datasheet
Cat. No. ABIN400577
Application IP, Purif, AC
Quantity 2 mL
Delivery 4 to 6 Days
Datasheet Datasheet

Mammalian immunoglobulin (Ig) can be purified from cell culture media, ascites and serum samples. These antibody-protein bound beads offer a convenient method to achieve high purity and high recovery of monoclonal and polyclonal antibodies from various biological samples. Naturally, the binding specificities and affinities of these proteins would vary between source species and antibody subclass. The table below summarizes the antibody binding specificities of each antibody-binding protein for human, mouse and rat.

Protein A / G / L Binding Affinities for Immunoglobulins

Species Immunoglobulin Protein A Protein G Protein L
H IgG (normal) ++++ ++++ ++++
H IgG1 ++++ ++++ ++++
H IgG2 ++++ ++++ ++++
H IgG3 - ++++ ++++
H IgG4 ++++ ++++ ++++
H IgM - - ++++
H IgA - - ++++
H IgE - - ++++
H IgD - - ++++
H Fab ++ ++ ++++
M IgG1 + ++++ ++++
M IgG2a ++++ ++++ ++++
M IgG2b +++ ++++ ++++
M IgG3 ++ +++ ++++
R IgG1 - + ++++
R IgG2a - ++++ ++++
R IgG2b - ++ ++++
R IgG2c + ++ ++++

Note: H = human, M = mouse, R = rat

Rene von der Forst
Rene von der Forst, MSE
Marketing and E-Commerce Manager

Master of Science in engineering. 12+ years of experience in marketing and e-commerce in the life science sector.

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