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GFP-Traps®, Chromobodies®, boosters and more!

What is a nanobody?

Nanobodies are tiny, recombinantly produced antigen binding VHH fragments, derived from the Alpaca heavy chain IgG antibody (HCAb).

Ig Forms

Pictured Bottom Row: The VHH domain represents the absolute smallest antibody fragment necessary to specifically bind an antigen with exceptionally high affinity.

When compared to traditional monoclonal or polyclonal antibodies, nanobodies are:

  • Smaller
  • More consistent
  • Higher affinity
  • Easier to use

Nanobodies are offered in a variety of formats, including Nano-Traps, Nano-Boosters, and Chromobodies® .

ChromoTek nanobodies have many superior performance characteristics:

Characteristic Monoclonal Antibody Poylclonal Antibody Nanobody
Size ~150 kDa unconjugated (IgG) ~150 kDa unconjugated (IgG) Only ~15 kDa unconjugated
Binding Affinity Medium to High
(nM - μM Kd values)
Low to Medium
(μM Kd values)
Consistently High
(pM - nM Kd values)
Lot-to-Lot Consistency High - Hybridoma cell line production can suffer from "drift" Very Low - Produced in live animals - significant variance between lots and bleeds Very High - Recombinantly produced from clonal DNA - same product every time!

How can I use a nanobody?

In short, nearly any way you please!

Nanobodies come in a variety of formats. They are raised against a fairly diverse range of antigens, and are thus highly versatile tools, capable of being employed in a variety of different situations.

The most common uses for a nanobody are experiments that you are likely already familiar with, like immunoprecipitation, or fluorescence microscopy.

There are also a handful of applications where use of a nanobody is feasible, but use of a conventional antibody would be impractical or impossible.

ChromoTek nanobodies are highly versatile reagents:

Application ChromoTek Advantages
Immunoprecipitation Nano-Traps Faster, cleaner, more consistent IPs without heavy & light chain contamination
Live Cell Imaging Chromobodies® Immunolabel intracellular structures in live cells simply and efficiently
Fluorescent Protein Enhancement Nano-Boosters Reactivate dead fluorescent proteins, or enhance the brightness & photostability of GFP or RFP

What is a Nano-Trap?

Nano-Traps are nanobodies that have been conjugated to agarose or magnetic beads to facilitate cleaner, faster, simpler immunoprecipitations.

Nano-Traps are available in a variety of formats, and target a variety of common tags, including Green Fluorescent Protein (GFP), Red Fluorescent protein (RFP), Myc, GST, & MBP.

Nano-Traps are also available for DNA methyltransferase (Dnmt-1), p53, and PARP-1 for immunoprecipitation of untagged, endogenously expressed protein

Ig Forms

Nano-Traps allow you to perform IP experiments with exceptionally high yield, and without contamination from antibody heavy or light chains.

In fact, the GFP-Trap® (pictured above) has already been cited in more than 400 peer reviewed publications. Try one today, and find out why they are so popular!

Nano-Traps significantly improve conventional IP protocols.

  • Faster (roughly half the time required for an antibody IP)
  • Cleaner (no heavy or light chain antibody contamination)
  • More consistent (recombinant reagents for the same interaction every time)
  • Higher yield (picomolar to nanomolar affinity constants for GFP and RFP-traps®)
Three forms of Nano-Traps

In order to facilitate the most versatility in IP methodology, all nano-traps come conjugated to agarose beads, ferromagnetic beads, or agarose/magnetic beads.

Researchers in the US and Western Europe get next-business day delivery on GFP and RFP-traps purchased at antibodies-online

Nano-Traps available at antibodies-online:

* GFP-traps will also recognize most GFP-derivatives, like eGFP and YFP
** RFP-traps will also recognize most Discosoma based RFP derivatives, like dsRed and mCherry
Antigen (A)-Type Nano-Trap (MA)-Type Nano-Trap
Green Fluorescent Protein (GFP)* ABIN509397 ABIN1889488
Red Fluorescent Protein (RFP)** ABIN509408 ABIN2452223
Red Fluorescent Protein (RFP)** ABIN509408 ABIN2452223
mNeonGreen ABIN5608490 ABIN5608496
Myc ABIN2559732 ABIN4368238
Glutathione S-transferase (GST) ABIN2656273 Please Inquire
Maltose Binding Protein(MBP) ABIN2870876 Please Inquire
p53 (N-terminal Epitope) ABIN2870929 Please Inquire
p53 (C-terminal Epitope) ABIN2870874 Please Inquire
Poly ADP-Ribose Polymerase (PARP1) ABIN2870878 Please Inquire

What is a Nano-Booster?

Nano-Boosters use the power of the nanobody to enhance, modulate, or reactivate weak fluorescent signal from a fluorescent protein.

A Nano-Booster is composed of a nanobody, targeting GFP or RFP, and conjugated to an ultra-bright ATTO dye.

Enhanced GFP signal with the ATTO-488 GFP-booster

The natural fluorescent properties of GFP are enhanced by the supebright ATTO-488 dye coupled to ABIN509419 the GFP-Booster®.

ATTO dyes were specifically chosen for use with Nano-Booster due to their exceptionally bright signal, best-in-class photostability, and narrow Stokes-Shift.

Next-generation imaging techniques like super-resolution microscopy require bright, photostable fluorescent probes.

Nano-Boosters can provide the "signal-boost" required to make fluorescent proteins like GFP bright and stable for super resolution microscopy.

antibodies-online offers the following Nano-Boosters:

Conjugate Dye GFP-Booster RFP-Booster
ATTO-488 ABIN509419 Please Inquire
ATTO-594 ABIN2452219 ABIN1082216
ATTO-647N ABIN2870880 ABIN2745402
Alexa Fluor® 647 ABIN6810549 Please Inquire

What is a Chromobody®?

Chromobodies® are novel tools for live-cell intracellular fluorescent labeling.

Chromobodies® are fusion proteins that contain both a nanobody targeting your protein of interest, and a bright, photostable fluorescent protein.

Because nanobodies are produced from recombinant DNA, you can generate Chromobodies® directly, right inside the cell you want to label. No fussing with messy, frustrating, or time consuming fixation or labeling protocols!

Simply start with the appropriate Chromobody® plasmid, transfect it into a compatible cell line, and utilize persistent, intracellular immunolabeling in live cells!

Actin Chromobody Staining Example

Chromobodies® use your own cells to produce the nanobodies that will label your protein of interest.

No fixation or additional staining steps are necessary. Finally, live-cell intracellular immunolabeling is a viable strategy!

Chromobody® plasmids are available for the following targets:

Antigen Subcellular Localization ABIN
Actin Cytosol ABIN1478361
Lamin Nuclear Envelope ABIN1478367
Poly ADP-Ribose Polymerase 1(PARP1) Nucleus, Mitochondria ABIN2745398
DNA methyltransferase 1 (Dnmt-1) Nucleus ABIN1478365
Vimentin Cytosol ABIN3071447
Ryan E. Robinson, Ph.D.
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