MBP-Catcher
Co-IP, IP, Purif, ChIP, RIP
Antibody
Agarose beads
90 μm
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- Target See all Maltose Binding Protein (MBP) products
- Maltose Binding Protein (MBP)
- Reactivity
- E. coli
- Host
- Camelid (Camelidae)
- Application
- Protein Complex Immunoprecipitation (Co-IP), Immunoprecipitation (IP), Purification (Purif), Chromatin Immunoprecipitation (ChIP), RNA-Binding Protein Immunoprecipitation (RIP)
- Sample Type
- Cell Extracts
- Specificity
- The Antibody with Clone 1G5 recognizes E.coli maltose-binding protein (MBP)
- Characteristics
- MBP-Catcher is based on a high-affinity single-domain antibody (sdAb) that is covalently immobilized on 4 % cross-linked agarose beads. The innovative, oriented and selective attachment via a flexible linker guarantees a high accessibility of the sdAbs and largely eliminates batch-to-batch variations. Due to the single-chain nature of sdAbs and their covalent attachment, no "leakage" of light and heavy chains from IgGs is observed during elution with SDS sample buffer. MBP-Catcher thus features high affinity and superior capacity for MBP fusion proteins while showing negligible non-specific background. MBP-Catcher is compatible not only with physiological buffers but also with high stringency buffers. MBP-Catcher thus provides great freedom to adjust the binding and washing conditions to the experimental needs.
- Components
- 4 % cross-linked agarose (bead size 50-150 μm) with covalently immobilized single-domain antibody
- Material not included
- wash buffers, columns, tubes
- Bead Ligand
- Antibody
- Bead Matrix
- Agarose beads
- Bead Size
- 90 μm
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- Application Notes
- 200 μL slurry for up to 10 reactions
- Comment
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Capacity: > 2.5 μg MBP per μl of packed beads (i.e. 2 μl of slurry)
- Restrictions
- For Research Use only
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- Buffer
- 50 % slurry in 20 % ethanol / PBS 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- Maltose Binding Protein (MBP)
- Alternative Name
- maltose binding protein, MBP (MBP Products)
- Background
- Maltose-binding protein (MBP) is encoded by the malE gene from the gram-negative bacterium Escherichia coli. When expressed as a fusion protein it boosts the expression of the fusion partner and is therefore a common entity in bacterial expression vectors
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