Epitope Tag Antibodies, ELISA Kits, Epitope Tags

A protein tag is a peptide sequence or carbohydrate which is genetically grafted onto a recombinant protein. The tag acts as an antigen that is recognized by an antibody. Tags fullfill various purposes when attached to proteins.

What are epitope tags?

Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. The technique of combining a recombinant protein with an epitope tag using genetic engineering is referred to as tagging. Epitope Tags are useful tools for the detection of proteins, especially when specific antibodies are lacking for example for newly discovered proteins and low abundant proteins. The sequence is often based on viral gene structures, which explains their high immunoreactivity.

Tag classes and their purpose

Affinity tags ease purification of the protein from their crude biological source. Solubilization tags assist in proper folding of the protein in chaperone-deficient species. Some affinity tags have a dual role as a solubilization agent, such as MBP, and GST. Fluorescence tags such as GFP are used to give visual readout on a protein and they can verify whether a protein is correctly folded

Affinity tags ease purification of the protein from their crude biological source. Solubilization tags assist in proper folding of the protein in chaperone-deficient species. Some affinity tags have a dual role as a solubilization agent, such as MBP, and GST. Fluorescence tags such as GFP are used to give visual readout on a protein and are used for cell tracking and cellular localization experiments. Tags such as c-my, HA and DYKDDDDK on the other hand are mainly used in pull-down/immunoprecipitation experiments to verify and identify protein-protein interactions.

antibodies-online offers a comprehensive selection of powerful, high-quality epitope tag antibodies and anti-reporter protein antibodies. Browse the list below to find the antibody against the tag you need.

Available tag-Antibodies