Goat anti-Guinea Pig IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN101281

Supplier Product No.: 606-103-129

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Guinea Pig

Host: Goat

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Supplier Product No.: 606-103-129

Supplier: Rockland

Purpose: Guinea Pig IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Cross-Reactivity (Details): Minimal crossreactivity against Bv Ch Gt Ham Hs Hu Ms Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum.  No reaction was observed against Bovine, Chicken, Goat, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins.

Characteristics: Anti-Guinea Pig IgG Fluorescein Antibody generated in goat detects guinea pig IgG.

Purification: Anti-Guinea Pig IgG (H&L) peroxidase conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Guinea Pig IgG (H&L) was produced by repeated immunization with Guinea Pig IgG whole molecule in goat.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Application Note: Anti-Guinea Pig IgG HRP has been tested by ELISA and western blot and is suitable for immunoblotting, western blot, dot blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:100,000 - 1:200,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Qi, Schmöhl, Li, Qian, Tabler, Bennewitz, Sticht, Morgenstern, Fleming, Volk, Hausser, Heidenreich, Hell, Nawroth, Kroll: "Reduced Acrolein Detoxification in akr1a1a Zebrafish Mutants Causes Impaired Insulin Receptor Signaling and Microvascular Alterations." in: Advanced science (Weinheim, Baden-Wurttemberg, Germany), pp. e2101281, (2021) (PubMed).

Duthie, Pena, Khandhar, Picone, MacMIllen, Truman, Adams, Reed: "Development of LepReact, a defined skin test for paucibacillary leprosy and low-level M. leprae infection." in: Applied microbiology and biotechnology, Vol. 104, Issue 9, pp. 3971-3979, (2020) (PubMed).

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.