Rabbit anti-Cow IgG (Whole Molecule) Antibody (Biotin) - Preadsorbed
WB, IF, IHC (fro), EIA
Polyclonal
Biotin
-
- Target See all IgG products
- IgG
-
Binding Specificity
- Whole Molecule
-
Reactivity
- Cow
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- Biotin
-
Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
- Characteristics
- Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
- Bovine IgG whole molecule
-
-
- Application Notes
-
ImmunoblottingELISAImmunohistochemistryImmunomicroscopyas well as other antibody based assays using streptavidin or avidin conjugates requiringlot-to-lot consistency. This product has been assayed against 1.0 μg of Bovine IgG in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 2.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) sodium azide as preservative. N-Hydroxysuccinimide Ester (BAC).
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Dilute only prior to immediate use
- Storage
- 4 °C
- Storage Comment
- Store vial at 2-8 °C short term only.
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
- Synonyms: Bovine Immunoglobulin G
-
You are here:
