Goat anti-Ferret IgM (Chain mu) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN117106

Quick Overview for Goat anti-Ferret IgM (Chain mu) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN117106)

Target: IgM

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Product Details

Binding Specificity: Chain mu

Reactivity: Ferret

Host: Goat

Purification: Preadsorption: Solid phase absorption

Immunogen: Ferret IgM µ chain

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry aswell as other phosphatase-antibody based enzymatic assays requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Ferret IgM in astandard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,500 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: 0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details

Target: IgM

Target Type: Antibody

Background: Synonyms: Ferret Immunoglobulin M