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Goat anti-Human Ig (Chain lambda), (Light Chain) Antibody (FITC)

FACS, IF, IEP, IM Polyclonal FITC F(ab')2 fragment
Catalog No. ABIN117721

Quick Overview for Goat anti-Human Ig (Chain lambda), (Light Chain) Antibody (FITC) (ABIN117721)

Target

Ig

Fragment

F(ab')2 fragment

Clonality

  • 116
  • 95
Polyclonal

Conjugate

  • 53
  • 25
  • 23
  • 22
  • 18
  • 14
  • 12
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
FITC

Application

  • 166
  • 132
  • 95
  • 68
  • 57
  • 26
  • 22
  • 12
  • 12
  • 7
  • 6
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoelectrophoresis (IEP), Immunomicroscopy (IM)
  • Binding Specificity

    • 79
    • 78
    • 62
    • 34
    • 4
    • 2
    Chain lambda, Light Chain

    Reactivity

    • 114
    • 62
    • 19
    • 17
    • 9
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 96
    • 77
    • 38
    • 18
    • 3
    • 1
    • 1
    Goat

    Characteristics

    Absorption / Emission: 495 nm / 528 nm
    Molar Ratio: 4.0 moles FITC per mole of Goat IgG F(ab')2.

    Purification

    Immunoaffinity chromatography.

    Immunogen

    Human lambda (lambda light chain)
  • Application Notes

    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring extremely low background levels, absence ofF(c) mediated binding, lot-to-lot consistency, high titer and specificity.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Reconstitution

    Restore with 1.0 mL of deionized water (or equivalent).

    Concentration

    1.0 mg/mL (by UV absorbance at 280 nm)

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01 % (w/v) Sodium Azide as preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing. Dilute only prior to immediate use

    Storage

    4 °C/-20 °C

    Storage Comment

    Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
  • Target

    Ig

    Alternative Name

    Immunoglobulin
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