Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (PE)

Catalog No. ABIN118383

Quick Overview for Mouse anti-Human Ig (Chain lambda), (Light Chain) Antibody (PE) (ABIN118383)

Target: Ig

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS)

Clone: Mc24-IC6

Isotype: IgG1

Product Details

Binding Specificity: Chain lambda, Light Chain

Reactivity: Human

Host: Mouse

Purification: Affinity chromatography on Protein A

Immunogen: Purified human IgG. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Application Details

Application Notes: Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL distilled water

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: RT/4 °C/-20 °C

Storage Comment: Lyophilized: Store at 2-8 °C (preferably in a desiccator). Reconstituted: Aliquot and store at -20 °C.

Target Details

Target: Ig

Alternative Name: Immunoglobulin

Background: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5 kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L, Lambda, 0.4-4.2 mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).