Mouse anti-Rat IgG2a Antibody (FITC)

Catalog No. ABIN118438

Quick Overview for Mouse anti-Rat IgG2a Antibody (FITC) (ABIN118438)

Target: IgG2a

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS)

Clone: MARG2a-1

Isotype: IgG1

Product Details

Reactivity: Rat

Host: Mouse

Purification: Affinity Chromatography

Immunogen: Purified Rat immunoglobulins from the IR33 - anti horse Ferritin antibody. Spleen cells from immunised BALB/c mice were fused with cells of the PAI-0 mouse myeloma cell line.

Application Details

Application Notes: Flow Cytometry: 5 μg/mL, use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG2a

Target Type: Antibody

Background: Synonyms: Rat Immunoglobulin G2a