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Mouse anti-Rat IgM (Heavy Chain) Antibody (FITC)

FACS Monoclonal MARM-4 IgG1 FITC
Catalog No. ABIN118445

Quick Overview for Mouse anti-Rat IgM (Heavy Chain) Antibody (FITC) (ABIN118445)

Target

IgM

Clonality

  • 514
  • 111
Monoclonal

Conjugate

  • 122
  • 84
  • 74
  • 56
  • 33
  • 27
  • 27
  • 20
  • 13
  • 12
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 11
  • 9
  • 9
  • 9
  • 9
  • 9
  • 7
  • 6
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
FITC

Application

  • 351
  • 310
  • 306
  • 242
  • 166
  • 114
  • 91
  • 91
  • 64
  • 64
  • 34
  • 34
  • 29
  • 25
  • 22
  • 13
  • 9
  • 6
  • 5
  • 3
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

MARM-4

Isotype

IgG1
  • Binding Specificity

    • 121
    • 91
    • 34
    • 24
    • 7
    • 6
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain

    Reactivity

    • 223
    • 161
    • 88
    • 40
    • 30
    • 29
    • 28
    • 22
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 12
    • 4
    • 2
    • 1
    Rat

    Host

    • 348
    • 230
    • 92
    • 23
    • 14
    • 5
    • 2
    • 1
    • 1
    Mouse

    Purification

    Affinity chromatography

    Immunogen

    IR202 rat IgM myeloma protein. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
  • Application Notes

    Flow Cytometry: 10 μg/mL, use 50 μL of the suggested working dilution to label 10^6 cells in 100 μL.

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.

    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS containing 0.09 % Sodium Azide and 50 % Glycerol

    Preservative

    Sodium azide

    Precaution of Use

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Handling Advice

    Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
  • Target

    IgM

    Target Type

    Antibody

    Background

    Synonyms: Rat Immunoglobulin M
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