Mouse anti-Duck IgY (Fc Region), (Heavy Chain) Antibody

Catalog No. ABIN120328

Quick Overview for Mouse anti-Duck IgY (Fc Region), (Heavy Chain) Antibody (ABIN120328)

Target: IgY

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 16C7

Isotype: IgG1

Product Details

Binding Specificity: Fc Region, Heavy Chain

Reactivity: Duck

Host: Mouse

Purification: Affinity chromatography on Protein G

Immunogen: Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Application Details

Application Notes: Flow Cytometry. ELISA. Western Blot under non-reducing conditions.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgY

Alternative Name: IgG + IgY

Background: The duck IgY molecule, consisting of two heavy (H) and two light (L) chains, has been categorized as the 7.8S full-length IgY form and the 5.7S truncated IgY(-Fc) form. Studies have shown that the IgY(-Fc) molecule possesses the H chain Cu1 and Cu2 constant domains, but is lacking the Cu3 and Cu4 constant region terminal domains, all four of which are present in the full-length IgY molecule. Thus the structure of the IgY(-Fc) molecule resembles that of a F(ab)2 fragment of IgY.Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y