Mouse anti-Duck IgY (Light Chain) Antibody

Catalog No. ABIN120329

Quick Overview for Mouse anti-Duck IgY (Light Chain) Antibody (ABIN120329)

Target: IgY

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 14A3

Isotype: IgG1

Product Details

Binding Specificity: Light Chain

Reactivity: Duck

Host: Mouse

Characteristics: Double-staining with clone 14A3 and duck CD8 alpha clone Du CD8-1 (product code MCA2479 ), revealed the presence of a CD8high /14A3- cytotoxic T cell population and a CD8low/14A3+ B cell population in duck spleen and also revealed a high percentage of CD8+ /14A3+ cells in duck bursa. The recognition of B cells by 14A3 was confirmed using a recently developed Flag-tagged chicken B cell marker, known as chBAFF-Flag (B cell activating factor). This antibody recognises IgA in Pekin duck bile and can be used as a marker to identify B cells in both Pekin and Muscovy duck and geese studies. This antibody detects a band of approximately 25 kDa in duck cell lysates in Western blotting.

Purification: Affinity chromatography on Protein G

Immunogen: Purified Pekin duck yolk IgY. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.

Application Details

Application Notes: Flow Cytometry. ELISA. Western Blot.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgY

Alternative Name: IgG + IgY

Background: IgY Light Chain is shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.Synonyms: Chicken Immunoglobulin G, Chicken Immunoglobulin Y

Molecular Weight: 25 kDa