Mouse anti-Human IgG Antibody

Catalog No. ABIN120644

Quick Overview for Mouse anti-Human IgG Antibody (ABIN120644)

Target: IgG

Clonality: Monoclonal

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Clone: 4A11

Isotype: IgG2b

Product Details

Reactivity: Human

Host: Mouse

Purification: Precipitation Methods and Ion Exchange Chromatography.

Immunogen: Purified human IgG

Application Details

Application Notes: ELISA. Western blotting. Has been described for previous lots to work in Flow Cytometry and Immunofluorescence.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4 with 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Target Details

Target: IgG

Target Type: Antibody

Background: Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.Synonyms: Human Immunoglobulin G