Goat anti-Mouse IgG Antibody (Peroxidase (POD))

Catalog No. ABIN1720696

Product Details

Target: IgG

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Peroxidase (POD)

Application: ELISA, Western Blotting (WB), Dot Blot (DB)

Specificity: This peroxidase conjugated antibody is specific for mouse IgG and shows no cross-reactivity with mouse IgA, IgM and other animal species IgG proteins.

Purification: This antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG.

Application Details

Application Notes:

Conjugation: Goat anti-mouse IgG is conjugated to peroxidase by means of a method described by Wilson MB and Nakane PK (Reference: Wilson MB and Nakane PK. In Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, P215 (1978).)

Preparation of Diluent Buffer: Use 0.01M TBS or 0.01M PBS to dilute. See “Recommended Dilutions” below for details.

Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

Recommended Dilutions:

• Dot blot (Enhanced chemiluminescent coloration) 1:2000-4000
• WB (DAB coloration) 1:500-3000
• WB (Enhanced chemiluminescent coloration) 1:3000-10000
• Direct ELISA (TMB coloration) 1:20000-40000

Restrictions: For Research Use only

Handling

Handling Advice: Do NOT add Sodium Azide!

Storage: 4 °C

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody