Goat anti-Mouse IgG3 (Heavy Chain) Antibody (FITC)

Catalog No. ABIN1967638

Quick Overview for Goat anti-Mouse IgG3 (Heavy Chain) Antibody (FITC) (ABIN1967638)

Target: IgG3

Clonality: Polyclonal

Conjugate: FITC

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Immunofluorescence (IF)

Product Details

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 0.5 % of target signal.

Purification: Immunoaffinity purified

Immunogen: Mouse IgG3 heavy chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA, FLISA (1:10000 - 1:50000), Flo, IF (1:1000 - 1:5000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-Mouse IgG3 secondary antibody is suitable for Flow Cytometry, Immunohistochemistry and Immunofluorescence Microscopy, ELISA, western blotting as well as other anti IgG2b antibody based assays. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2 BSA 10 mg/mL, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details

Target: IgG3

Target Type: Antibody