Mouse anti-Dog IgE Antibody

Catalog No. ABIN2474291

Quick Overview for Mouse anti-Dog IgE Antibody (ABIN2474291)

Target: IgE

Clonality: Monoclonal

Application: ELISA, Western Blotting (WB)

Clone: E6-71A1

Isotype: IgG1

Product Details

Reactivity: Dog

Host: Mouse

Specificity: Mouse anti Dog IgE antibody, clone E6-71A1 recognises canine IgE. E6-2A1 does not crossreact with dog IgM, IgA, IgG1 or IgG2. Western blot analysis against affinity purified dog IgE using Mouse anti Dog IgE clone E6-71A1 demonstrates a single major band of 62-65kDa underreducing conditions.

Characteristics: Purified IgG

Purification: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Immunogen: Affinity purified IgE preparation from heavily parasitized and allergic dog serum.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline
Contains: 0.05% Sodium Azide (NaN3)

Preservative: Sodium azide

Storage: 4 °C/-20 °C

Storage Comment: Store at +4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as thismay denature the antibody. Should this product contain a precipitate we recommendmicrocentrifugation before use.

References

Ognjenovic, Milcic-Matic, Smiljanic, Vuckovic, Burazer, Popovic, Stanic-Vucinic, Velickovic: "Immunoproteomic characterization of Ambrosia artemisiifolia pollen allergens in canine atopic dermatitis." in: Veterinary immunology and immunopathology, Vol. 155, Issue 1-2, pp. 38-47, (2013) (PubMed).

Target Details

Target: IgE

Molecular Weight: Under reducing conditions the antibody detects a band of approximately 62-65 kDa in samples of affinity purified dog IgE.